Identification and characterization of a silent mutation in RNA binding domain of N protein coding gene from SARS-CoV-2

Reza Zolfaghari Emameh, Mahyar Eftekhari, Hassan Nosrati, Jalal Heshmatnia, Reza Falak

科研成果: 期刊稿件文章同行评审

1 引用 (Scopus)

摘要

Objective: This study describes the occurrence of a silent mutation in the RNA binding domain of nucleocapsid phosphoprotein (N protein) coding gene from SARS-CoV-2 that may consequence to a missense mutation by onset of another single nucleotide mutation. Results: In the DNA sequence isolated from severe acute respiratory syndrome (SARS-CoV-2) in Iran, a coding sequence for the RNA binding domain of N protein was detected. The comparison of Chinese and Iranian DNA sequences displayed that a thymine (T) was mutated to cytosine (C), so “TTG” from China was changed to “CTG” in Iran. Both DNA sequences from Iran and China have been encoded for leucine. In addition, the second T in “CTG” in the DNA or uracil (U) in “CUG” in the RNA sequences from Iran can be mutated to another C by a missense mutation resulting from thymine DNA glycosylase (TDG) of human and base excision repair mechanism to produce “CCG” encoding for proline, which consequently may increase the affinity of the RNA binding domain of N protein to viral RNA and improve the transcription rate, pathogenicity, evasion from human immunity system, spreading in the human body, and risk of human-to-human transmission rate of SARS-CoV-2.
源语言英语
期刊BMC Research Notes
14
1
DOI
已出版 - 1 十二月 2021

指纹 探究 'Identification and characterization of a silent mutation in RNA binding domain of N protein coding gene from SARS-CoV-2' 的科研主题。它们共同构成独一无二的指纹。

引用此