Development of pooled testing system for porcine epidemic diarrhoea using real-time fluorescent reverse-transcription loop-mediated isothermal amplification assay

  • Thi Ngan Mai (投稿者)
  • Van Diep Nguyen (投稿者)
  • Wataru Yamazaki (投稿者)
  • Tamaki Okabayashi (投稿者)
  • Shuya Mitoma (投稿者)
  • Kosuke Notsu (投稿者)
  • Yuta Sakai (投稿者)
  • R. Yamaguchi (投稿者)
  • Junzo Norimine (投稿者)
  • Satoshi Sekiguchi (投稿者)

数据集

描述

Abstract Background Porcine epidemic diarrhoea (PED) is an emerging disease in pigs that causes massive economic losses in the swine industry, with high mortality in suckling piglets. Early identification of PED virus (PEDV)-infected herd through surveillance or monitoring strategies is necessary for mass control of PED. However, a common working diagnosis system involves identifying PEDV-infected animals individually, which is a costly and time-consuming approach. Given the above information, the thrusts of this study were to develop a real-time fluorescent reverse transcription loop-mediated isothermal amplification (RtF-RT-LAMP) assay and establish a pooled testing system using faecal sample to identify PEDV-infected herd. Results In this study, we developed an accurate, rapid, cost-effective, and simple RtF- RT-LAMP assay for detecting the PEDV genome targeting M gene. The pooled testing system using the RtF-RT-LAMP assay was optimized such that a pool of at least 15 individual faecal samples could be analysed. Conclusions The developed RtF-RT-LAMP assay in our study could support the design and implementation of large-scaled epidemiological surveys as well as active surveillance and monitoring programs for effective control of PED.
可用的日期1 一月 2018
出版商Figshare

引用此

Mai, T. N. (投稿者), Nguyen, V. D. (投稿者), Yamazaki, W. (投稿者), Okabayashi, T. (投稿者), Mitoma, S. (投稿者), Notsu, K. (投稿者), Sakai, Y. (投稿者), Yamaguchi, R. (投稿者), Norimine, J. (投稿者), Sekiguchi, S. (投稿者) (1 一月 2018). Development of pooled testing system for porcine epidemic diarrhoea using real-time fluorescent reverse-transcription loop-mediated isothermal amplification assay. Figshare. 10.6084/m9.figshare.c.4116491.v1