1H, 13C and 15N chemical shift assignment of the stem-loop 5a from the 5′-UTR of SARS-CoV-2

Robbin Schnieders, Stephen A. Peter, Elnaz Banijamali, Magdalena Riad, Nadide Altincekic, Jasleen Kaur Bains, Betül Ceylan, Boris Fürtig, J. Tassilo Grün, Martin Hengesbach, Katharina F. Hohmann, Daniel Hymon, Bozana Knezic, Andreas Oxenfarth, Katja Petzold, Nusrat S. Qureshi, Christian Richter, Judith Schlagnitweit, Andreas Schlundt, Harald SchwalbeElke Stirnal, Alexey Sudakov, Jennifer Vögele, Anna Wacker, Julia E. Weigand, Julia Wirmer-Bartoschek, Jens Wöhnert

Research output: Contribution to journalArticlepeer-review

Abstract

The SARS-CoV-2 (SCoV-2) virus is the causative agent of the ongoing COVID-19 pandemic. It contains a positive sense single-stranded RNA genome and belongs to the genus of Betacoronaviruses. The 5′- and 3′-genomic ends of the 30 kb SCoV-2 genome are potential antiviral drug targets. Major parts of these sequences are highly conserved among Betacoronaviruses and contain cis-acting RNA elements that affect RNA translation and replication. The 31 nucleotide (nt) long highly conserved stem-loop 5a (SL5a) is located within the 5′-untranslated region (5′-UTR) important for viral replication. SL5a features a U-rich asymmetric bulge and is capped with a 5′-UUUCGU-3′ hexaloop, which is also found in stem-loop 5b (SL5b). We herein report the extensive H, C and N resonance assignment of SL5a as basis for in-depth structural studies by solution NMR spectroscopy. 1 13 15
Original languageEnglish
JournalBiomolecular NMR Assignments
Volume15
Issue number1
DOIs
StatePublished - 1 Apr 2021

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