The nucleocapsid protein (N) is a major structural protein of coronaviruses. The N protein of bat SARS-like coronavirus (SL-CoV) has a high similarity with that of SARS-CoV. In this study, the SL-CoV N protein was expressed in Escherichia coli, purified and used as antigen. An Indirect Enzyme-Linked Immunosorbent Assay (indirect ELISA) was developed for detection of SARS- or SL-CoV infections in bat populations. The detection of 573 bat sera with this indirect ELISA demonstrated that SL-CoVs consistently circulate in Rhinilophus species, further supporting the proposal that bats are natural reservoirs of SL-CoVs. This method uses 1-2 μl of serum sample and can be used for preliminary screening of infections by SARS- or SL-CoV with a small amount of serum sample. © Wuhan Institute of Virology, CAS and Springer-Verlag GmbH 2009.
Yuan, J. F., Li, Y., Zhang, H. J., Zhou, P., Ke, Z. H., Zhang, Y. Z., & Shi, Z. L. (2009). Indirect enzyme-linked immunosorbent assay based on the nucleocapsid protein of SARS-like coronaviruses. Virologica Sinica, 24(2). https://doi.org/10.1007/s12250-009-3037-0