Indirect enzyme-linked immunosorbent assay based on the nucleocapsid protein of SARS-like coronaviruses

Jun Fa Yuan, Yan Li, Hua Jun Zhang, Peng Zhou, Zhen Hua Ke, Yun Zhi Zhang, Zheng Li Shi

Research output: Contribution to journalArticle

Abstract

The nucleocapsid protein (N) is a major structural protein of coronaviruses. The N protein of bat SARS-like coronavirus (SL-CoV) has a high similarity with that of SARS-CoV. In this study, the SL-CoV N protein was expressed in Escherichia coli, purified and used as antigen. An Indirect Enzyme-Linked Immunosorbent Assay (indirect ELISA) was developed for detection of SARS- or SL-CoV infections in bat populations. The detection of 573 bat sera with this indirect ELISA demonstrated that SL-CoVs consistently circulate in Rhinilophus species, further supporting the proposal that bats are natural reservoirs of SL-CoVs. This method uses 1-2 μl of serum sample and can be used for preliminary screening of infections by SARS- or SL-CoV with a small amount of serum sample. © Wuhan Institute of Virology, CAS and Springer-Verlag GmbH 2009.
Original languageEnglish
JournalVirologica Sinica
Volume24
Issue number2
DOIs
StatePublished - 29 Apr 2009

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