Characterization of an internal ribosome entry site within mRNA 5 of murine hepatitis virus

M. Jendrach, V. Thiel, S. Siddell

Research output: Contribution to journalArticle

Abstract

The unique region of mRNA 5 of murine hepatitis virus contains two open reading frames, ORF 5a and ORF 5b. The downstream ORF 5b encodes the envelope (E) protein, an integral membrane protein of the virus. We have shown previously that the expression of ORF 5b is mediated by the internal entry of ribosomes. In the experiments reported here, we have used the in vitro translation of synthetic mRNAs to identify the region of mRNA 5 that mediates internal ribosome entry. Our results show that the 5' border of the MHV mRNA 5 IRES element is located between nucleotides 227 and 244 in ORF 5a, while the 3' border is located between nucleotides 140 and 172 in ORF 5b. The MHV mRNA 5 IRES element, therefore, contains not more than 280 nucleotides and encompasses the ORF 5b initiation codon. As evidenced by electrophoretic mobility shift assays, the IRES element of mRNA 5 interacts specifically with protein factors present in an L-cell lysate.
Original languageEnglish
JournalArchives of Virology
Volume144
Issue number5
DOIs
StatePublished - 1 Jul 1999

Fingerprint Dive into the research topics of 'Characterization of an internal ribosome entry site within mRNA 5 of murine hepatitis virus'. Together they form a unique fingerprint.

  • Cite this