Identification and characterization of a silent mutation in RNA binding domain of N protein coding gene from SARS-CoV-2

Reza Zolfaghari Emameh, Mahyar Eftekhari, Hassan Nosrati, Jalal Heshmatnia, Reza Falak

Sortida de recerca: Articlerevisió per companys

1 Citació (Scopus)

Resum

Objective: This study describes the occurrence of a silent mutation in the RNA binding domain of nucleocapsid phosphoprotein (N protein) coding gene from SARS-CoV-2 that may consequence to a missense mutation by onset of another single nucleotide mutation. Results: In the DNA sequence isolated from severe acute respiratory syndrome (SARS-CoV-2) in Iran, a coding sequence for the RNA binding domain of N protein was detected. The comparison of Chinese and Iranian DNA sequences displayed that a thymine (T) was mutated to cytosine (C), so “TTG” from China was changed to “CTG” in Iran. Both DNA sequences from Iran and China have been encoded for leucine. In addition, the second T in “CTG” in the DNA or uracil (U) in “CUG” in the RNA sequences from Iran can be mutated to another C by a missense mutation resulting from thymine DNA glycosylase (TDG) of human and base excision repair mechanism to produce “CCG” encoding for proline, which consequently may increase the affinity of the RNA binding domain of N protein to viral RNA and improve the transcription rate, pathogenicity, evasion from human immunity system, spreading in the human body, and risk of human-to-human transmission rate of SARS-CoV-2.
Idioma originalEnglish
RevistaBMC Research Notes
Volum14
Número d'incidència1
DOIs
Estat de la publicacióPublished - 1 de des. 2021

Empremta digital Navegar pels temes de recerca de 'Identification and characterization of a silent mutation in RNA binding domain of N protein coding gene from SARS-CoV-2'. Junts formen una empremta única.

Citeu això