ACE2 expression is related to the interferon response in airway epithelial cells but is that functional for SARS-CoV-2 entry?

Carolina Scagnolari, Camilla Bitossi, Agnese Viscido, Federica Frasca, Giuseppe Oliveto, Mirko Scordio, Laura Petrarca, Enrica Mancino, Raffaella Nenna, Elisabetta Riva, Corrado De Vito, Fabio Midulla, Guido Antonelli, Alessandra Pierangeli

Sortida de recerca: Articlerevisió per companys

1 Citació (Scopus)

Resum

In vitro interferon (IFN)α treatment of primary human upper airway basal cells has been shown to drive ACE2 expression, the receptor of SARS-CoV-2. The protease furin is also involved in mediating SARS‐CoV‐2 and other viral infections, although its association with early IFN response has not been evaluated yet. In order to assess the in vivo relationship between ACE2 and furin expression and the IFN response in nasopharyngeal cells, we first examined ACE2 and furin levels and their correlation with the well-known marker of IFNs’ activation, ISG15, in children (n = 59) and adults (n = 48), during respiratory diseases not caused by SARS-CoV-2. A strong positive correlation was found between ACE2 expression, but not of furin, and ISG15 in all patients analyzed. In addition, type I and III IFN stimulation experiments were performed to examine the IFN-mediated activation of ACE2 isoforms (full-length and truncated) and furin in epithelial cell lines. Following all the IFNs treatments, only the truncated ACE2 levels, were upregulated significantly in the A549 and Calu3 cells, in particular by type I IFNs. If confirmed in vivo following IFNs’ activation, the induction of the truncated ACE2 isoform only would not enhance the risk of SARS‐CoV‐2 infection in the respiratory tract.
Idioma originalEnglish
RevistaCytokine
Volum140
DOIs
Estat de la publicacióPublished - 1 d’abr. 2021

Empremta digital Navegar pels temes de recerca de 'ACE2 expression is related to the interferon response in airway epithelial cells but is that functional for SARS-CoV-2 entry?'. Junts formen una empremta única.

Citeu això